Articoli scientifici - novità in oftalmologia

(articoli presenti: 1144)

Adaptive-optics SLO imaging combined with widefield OCT and SLO enables precise 3D localization of fluorescent cells in the mouse retina

Abstract: Adaptive optics scanning laser ophthalmoscopy (AO-SLO) has recently been used to achieve exquisite subcellular resolution imaging of the mouse retina. Wavefront sensing-based AO typically restricts the field of view to a few degrees of visual angle. As a consequence the relationship between AO-SLO data and larger scale retinal structures and cellular patterns can be difficult to assess.


 The retinal vasculature affords a largescale 3D map on which cells and structures can be located during in vivo imaging. Phase-variance OCT (pv-OCT) can efficiently image the vasculature with near-infrared light in a label-free manner, allowing 3D vascular reconstruction with high precision. We combined widefield pvOCT and SLO imaging with AO-SLO reflection and fluorescence imaging to localize two types of fluorescent cells within the retinal layers: GFPexpressing microglia, the resident macrophages of the retina, and GFPexpressing cone photoreceptor cells. We describe in detail a reflective afocal AO-SLO retinal imaging system designed for high resolution retinal imaging in mice. The optical performance of this instrument is compared to other state-of-the-art AO-based mouse retinal imaging systems. 

The spatial and temporal resolution of the new AO instrumentation was characterized with angiography of retinal capillaries, including blood-flow velocity analysis. Depth-resolved AO-SLO fluorescent images of microglia and cone photoreceptors are visualized in parallel with 469 nm and 663 nm reflectance images of the microvasculature and other structures. Additional applications of the new instrumentation are discussed.


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Adaptive-optics SLO imaging combined with widefield OCT and SLO enables precise 3D localization of fluorescent cells in the mouse retina


Area Tematica : oct

AngioVue Optovue


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AngioVue Optovue


Area Tematica : oct

Swept-source optical coherence tomography angiography for choroidal neovascularization after bevacizumab and photodynamic Q3 therapy


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Swept-source optical coherence tomography angiography for choroidal neovascularization after bevacizumab and photodynamic Q3 therapy


Area Tematica : oct

Wideband Electrically Pumped 1050-nm MEMS-Tunable VCSEL for Ophthalmic Imaging


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Wideband Electrically Pumped 1050-nm MEMS-Tunable VCSEL for Ophthalmic Imaging


Area Tematica : oct

Retinal, anterior segment and full eye imaging using ultrahigh speed swept source OCT with vertical-cavity surface emitting lasers


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Retinal, anterior segment and full eye imaging using ultrahigh speed swept source OCT with vertical-cavity surface emitting lasers


Area Tematica : oct

ANGIOVUE OCT-Angiography (OCTA) system


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ANGIOVUE OCT-Angiography (OCTA) system


Area Tematica : oct

Swept-Source OCT Angiography: SS OCT AngioTM


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Swept-Source OCT Angiography: SS OCT AngioTM


Area Tematica : oct

Statistical analysis of motion contrast in optical coherence tomography angiography


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Statistical analysis of motion contrast in optical coherence tomography angiography


Area Tematica : oct

A Review of Algorithms for Segmentation of Retinal Image Data Using Optical Coherence Tomograph


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A Review of Algorithms for Segmentation of Retinal Image Data Using Optical Coherence Tomograph


Area Tematica : oct

Mathematical modeling and glaucoma: the need for an individualized approach to risk assessment


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Mathematical modeling and glaucoma: the need for an individualized approach to risk assessment


Area Tematica : glaucoma

Optical Coherence Tomography Angiography in Retinal Vascular Diseases and Choroidal Neovascularization


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Optical Coherence Tomography Angiography in Retinal Vascular Diseases and Choroidal Neovascularization


Area Tematica : oct

Combined eye gel containing sodium hyaluronate and xanthan gum for the treatment of the corneal epithelial defect after pterygium surgery


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Combined eye gel containing sodium hyaluronate and xanthan gum for the treatment of the corneal epithelial defect after pterygium surgery


Area Tematica : congiuntiva

ARVO 2015 Annual Meeting Abstracts


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ARVO 2015 Annual Meeting Abstracts


Area Tematica : oct

Improved microcirculation imaging of human skin in vivo using optical microangiography with a correlation mapping mask


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Improved microcirculation imaging of human skin in vivo using optical microangiography with a correlation mapping mask


Area Tematica : oct

Quantitative optical coherence tomography angiography of vascular abnormalities in the living human eye


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Quantitative optical coherence tomography angiography of vascular abnormalities in the living human eye


Area Tematica : oct

Optimization of the split-spectrum amplitude-decorrelation angiography algorithm on a spectral optical coherence tomography system


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Optimization of the split-spectrum amplitude-decorrelation angiography algorithm on a spectral optical coherence tomography system


Area Tematica : oct

Automated choroidal neovascularization detection algorithm for optical coherence tomography angiography


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Automated choroidal neovascularization detection algorithm for optical coherence tomography angiography


Area Tematica : oct

Swept-source OCT Angiography of the Retinal Vasculature using Intensity Differentiation Based OMAG Algorithms


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Swept-source OCT Angiography of the Retinal Vasculature using Intensity Differentiation Based OMAG Algorithms


Area Tematica : oct

Complex differential variance algorithm for optical coherence tomography angiography


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Complex differential variance algorithm for optical coherence tomography angiography


Area Tematica : oct

Technologies for Analyzing the Optic Disc, RNFL, and Macula

Optical Coherence Tomography By Lindsey S. Folio, BSc; Gadi Wollstein, MD; and Joel S. Schuman, MD


 Glaucoma can be characterized as a slowly progressing optic neuropathy that damages the retinal ganglion cells (RGCs) and their axons. This damage causes thinning of the retinal nerve fiber layer (RNFL), leads to cupping of the optic nerve head (ONH), and usually results in observable visual field loss. Because glaucomatous damage is irreversible, early detection is crucial for effective treatment through medical or surgical methods to halt further progression and preserve functional vision.

 Studies have shown that up to half of the RGCs could be damaged before defects are reported on the visual field.1 A more resolute, objective method of identifying glaucoma is therefore needed for at-risk patients. 


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Technologies for Analyzing the Optic Disc, RNFL, and Macula


Area Tematica : glaucoma

ImageJ User Guide


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ImageJ User Guide


Area Tematica : oct

Angio OCT, Dye Less Angiography, A New Approach of Age Related Macular Degeneration (ARMD)


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Angio OCT, Dye Less Angiography, A New Approach of Age Related Macular Degeneration (ARMD)


Area Tematica : oct

Noninvasive Visualization and Analysis of the Human Parafoveal Capillary Network Using Swept Source OCT Optical Microangiography


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Noninvasive Visualization and Analysis of the Human Parafoveal Capillary Network Using Swept Source OCT Optical Microangiography


Area Tematica : oct

Basic Technology and Clinical Applications of the Updated Model of Laser Speckle Flowgraphy to Ocular Diseases


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Basic Technology and Clinical Applications of the Updated Model of Laser Speckle Flowgraphy to Ocular Diseases


Area Tematica : oct

”Great diagnostic potential”


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”Great diagnostic potential”


Area Tematica : oct

ARVO 2015


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ARVO 2015


Area Tematica : oct

OCT angiography by absolute intensity difference applied to normal and diseased human retinas

Abstract: We compare four optical coherence tomography techniques for noninvasive visualization of microcapillary network in the human retina and murine cortex. We perform phantom studies to investigate contrast-tonoise ratio for angiographic images obtained with each of the algorithm. We show that the computationally simplest absolute intensity difference angiographic OCT algorithm that bases only on two cross-sectional intensity images may be successfully used in clinical study of healthy eyes and eyes with diabetic maculopathy and branch retinal vein occlusion. ©2015 Optical Society of America OCIS codes: (110.4500) Optical coherence tomography; (170.4470) Ophthalmology; (170.3880) Medical and biological imaging; (280.2490) Flow diagnostics. 


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OCT angiography by absolute intensity difference applied to normal and diseased human retinas


Area Tematica : oct

Small eyesdbig problems


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Small eyesdbig problems


Area Tematica : malformazioni rare

Image-guided femtosecond laser–assisted cataract surgery in Peters anomaly type 2


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Image-guided femtosecond laser–assisted cataract surgery in Peters anomaly type 2


Area Tematica : cataratta

Single suture iris-to-capsulorhexis fixation for in-the-bag intraocular lens subluxation


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Single suture iris-to-capsulorhexis fixation for in-the-bag intraocular lens subluxation


Area Tematica : cataratta

The X Factors: Three areas that will improve refractive cataract surgery outcomes


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The X Factors: Three areas that will improve refractive cataract surgery outcomes


Area Tematica : cataratta

Step by step: Clinical and practical implementation of laser-assisted cataract surgery


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Step by step: Clinical and practical implementation of laser-assisted cataract surgery


Area Tematica : cataratta

Crossing the finish line: Launching your premium technology practice


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Crossing the finish line: Launching your premium technology practice


Area Tematica : cataratta

ASCRS completes third annual Clinical Survey


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ASCRS completes third annual Clinical Survey


Area Tematica : difetti di vista

Innovating advanced treatments to increase compliance and improve outcomes for glaucoma patients: diagnostics, novel therapies, and surgical options


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Innovating advanced treatments to increase compliance and improve outcomes for glaucoma patients: diagnostics, novel therapies, and surgical options


Area Tematica : glaucoma

Driving laser vision correction outcomes, category growth, and confidence in today’s turbulent environment


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Driving laser vision correction outcomes, category growth, and confidence in today’s turbulent environment


Area Tematica : cataratta

What do the recent technological advancements for femto cataract surgery mean?


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What do the recent technological advancements for femto cataract surgery mean?


Area Tematica : cataratta

Elaborazioni Informatiche

La ricerca svolta nel periodo gennaio-settembre 2011 é composta dalle seguenti attività: 


1. Analisi dello stato dell’arte, in particolare del lavoro svolto nel biennio 2004-2006 dal gruppo coordinato dal Prof. Giovanni Frosoli del Dipartimento di Matematica Applicata dell’Università di Firenze. 

Nella sezione 2 presentiamo una rivisitazione critica di questo lavoro. 

2. Fase preliminare di studio della letteratura, delle tecniche e degli algoritmi esistenti nel campo dell’elaborazione di immagini digitali. 

3. Lavoro di consulenza nelle acquisizioni manuali delle immagini di prova, in particolare per quanto riguarda la calibrazione dei parametri di acquisizione. 

4. Scelta e sviluppo degli algoritmi per l’effettiva elaborazione delle immagini di prova a disposizione. Nella sezione 3 descriveremo in dettaglio i risultati conseguiti. 

5. Prima individuazione delle tecniche statistiche e degli algoritmi di classificazione. 

Nella sezione 4 concluderemo presentando i possibili approcci al momento ipotizzati al problema e definendo lo schema delle successive fasi del lavoro. 


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Elaborazioni Informatiche


Area Tematica : oct

A review of optical coherence tomography angiography (OCTA)


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A review of optical coherence tomography angiography (OCTA)


Area Tematica : oct

Scleretizzazione


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Scleretizzazione


Area Tematica : oct

Correlazioni Lineari


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Correlazioni Lineari


Area Tematica : oct

OCT Angiography: The Newest Frontier for the Revolutionary Technology


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OCT Angiography: The Newest Frontier for the Revolutionary Technology


Area Tematica : oct

Prospective Trial of Treat-and-Extend versus Monthly Dosing for Neovascular Age-Related Macular Degeneration


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Prospective Trial of Treat-and-Extend versus Monthly Dosing for Neovascular Age-Related Macular Degeneration


Area Tematica : maculopatie

Optical Coherence Tomography Angiography of peripapillary retinal blood flow response to Hyperoxia


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Optical Coherence Tomography Angiography of peripapillary retinal blood flow response to Hyperoxia


Area Tematica : oct

Swept-Source Angio OCT


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Swept-Source Angio OCT


Area Tematica : oct

Revue de la littérature 2014-2015Aangio-OCT


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Revue de la littérature 2014-2015Aangio-OCT


Area Tematica : oct

Methods and algorithms for optical coherence tomography-based angiography: a review and comparison


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Methods and algorithms for optical coherence tomography-based angiography: a review and comparison


Area Tematica : oct

Optical Microangiography: A Label Free 3D Imaging Technology to Visualize and Quantify Blood Circulations within Tissue Beds in vivo


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Optical Microangiography: A Label Free 3D Imaging Technology to Visualize and Quantify Blood Circulations within Tissue Beds in vivo


Area Tematica : oct

Comparison of Anterior Segment-Optical Coherence Tomography Parameters in Phacomorphic Angle Closure and Acute Angle Closure Eyes


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Comparison of Anterior Segment-Optical Coherence Tomography Parameters in Phacomorphic Angle Closure and Acute Angle Closure Eyes


Area Tematica : oct

Multifunctional 1050 nm Spectral Domain Oct System at 147 khz for Posterior Eye Imaging


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Multifunctional 1050 nm Spectral Domain Oct System at 147 khz for Posterior Eye Imaging


Area Tematica : oct

Ultra–Short-Term Reproducibility of Speckle-Noise Freed Fluid and Tissue Compartmentalization of the Choroid Analyzed by Standard OCT


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Ultra–Short-Term Reproducibility of Speckle-Noise Freed Fluid and Tissue Compartmentalization of the Choroid Analyzed by Standard OCT


Area Tematica : oct

Advances in Ophthalmologic Imaging


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Advances in Ophthalmologic Imaging


Area Tematica : oct
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